Biography of robert feulgent

He also succeeded in converting form A to B by using a commercial pancreatic juice to digest the polymer into fragments. The enzyme present in the pancreatic juice, responsible by the depolymerization, was termed nucleogelase by Feulgen; and from the same. Although modern molecular geneticists now appreciate the great importance of Feulgen's pioneering studies, at his own time little attention was given to his experimental work.

The Feulgen reaction is still in use as an effective staining technique in studies of chromosomes and in genetic cytochemistry assays. Click Here to Know about a Legend Dr. Abdul Kalam. Toggle navigation Menu. Social Discuss Sign Up Login. Robert Feulgen Popular Inventions. Home inventors Robert Feulgen. Robert Feulgen - Famous Inventor. About Inventor Robert Feulgen,who was lived between — was a German physician and chemist who, in , developed a method for staining DNA or otherwise called as the Feulgen stain.

Early Life and his Career Feulgen was born in Werden, Germany, into a working class family, Feulgen paved his way to the medical school through an outstanding student performance that opened to him the doors of the University of Freiburg at Breisgau in Another important Feulgen contribution to the understanding of the chemical e molecular structure of DNA came in through his studies of the nucleic acids in their undegraded state, which led him to conclude that nucleic acids were in fact molecular polymers formed by oligonucleotides, each comprised of the bases thymine, cytosine, adenine and guanine.

The enzyme present in the pancreatic juice, responsible by the depolymerization, was termed nucleogelase by Feulgen; and from the same commercial juice DNA depolymerase was isolated some years later. Follow Us on. Chennai Coimbatore Madurai Puducherry Trichy. Jodhpur Vijayawada Visakhapatnam Lucknow Nagpur. Interestingly, detection of DNA at the EM level showed some strange patterns in particular types of chromatin.

In mature sperm nuclei in mouse Biggiogera and other species Courtens et al. The reason for this behaviour lies in microheterogeneities of the protamine—protamine interaction Boutinard Rouelle-Rossier and Biggiogera rather than reflecting biological features. Furthermore, chromosomes and their characteristics were also studied with interesting findings related to their structure Liu et al.

For example, developing mouse embryos Fakan and Odartchenko were shown to exhibit a thin rim of DNA surrounding the prenucleolar bodies. Several reviews summarize in depth the wide spectrum of applications of OA in revealing chromatin architecture Biggiogera et al. By tracing the historical development and widespread adoption of the Feulgen reaction across various scientific domains, its enduring relevance in many still evolving fields becomes evident.

To date, many approaches based on the Feulgen reaction have been developed, most directed towards gaining a comprehensive understanding of chromatin organization through the evaluation of geometric, densitometric and textural features. By integrating diverse tools such as light microscopy and cytometry it has been possible to assess chromatin condensed areas among different organisms or conditions, exploring changes in chromatin density during different developmental stages, aging progression, and tumorigenesis for a review, see Mello and Vidal However, the Feulgen method, when supported by microscopy-based approaches, offers the possibility to compare morphological data with absorption analyses, therefore enabling the specific observation of variations in DNA distribution across nuclei and its intensity.

Comparison of these parameters between distinct specimens offers the opportunity to investigate between numerous chromatin organization patterns, therefore providing unexplored insights into epigenetics, a still expanding field of research. Alkan B, Koroglu-Aydin P The effects of smoking on genotoxic and histopathologic damage in exfoliated oral epithelial cells and the periodontium: a cross-sectional study.

Medicine Baltimore 8 :e Histochemie — Histochemistry — Biggiogera M Unusual ultrastructural features of chromatin in mouse sperm cells after DNA staining with osmium ammines. Bas Appl Histochem — CAS Google Scholar. Biggiogera M The Feulgen reaction at the electron microscopy level. Eur J Histochem. Biol Cell — J Cell Sci — Boutinard Rouelle-Rossier V, Biggiogera M Electron spectroscopic imaging and X-ray microanalysis of phosphorus in mouse sperm chromatin.

Eur J Histochem — PubMed Google Scholar. Bretschneider LH An electron microscopical study of bull sperm. Proc K Ned Akad Wet — Google Scholar. Eur J Histochem Skandinav Arch Physiol — Histochem Cell Biol — Cell Tissue Res — Ultrastructural evaluation of the Feulgen-like technique using osmium ammine. Reprod Nutr Dev — Cunningham L, Griffin AC, Luck JM Polyploidy and cancer; the desoxypentosenucleic acid content of nuclei of normal, precancerous, and neoplastic rat tissues.

J Gen Physiol — Genet Mol Biol — Article Google Scholar. J Invest Dermatol — J Ultrastruct Res — Derenzini M, Hernandez-Verdun D, Bouteille M Visualization of a repeating subunit organization in rat hepatocyte chromatin fixed in situ. Vet Ophthalmol — Duijndam WA, van Duijn P The influence of chromatin compactness on the stoichiometry of the Feulgen-Schiff procedure studied in model films.

Investigations on films containing condensed or swollen chicken erythrocyte nuclei. J Histochem Cytochem — New structures arise during the S phase and condense at prophase into «chromomeres» which fuse at prometaphase into mitotic chromosomes. Anat Rec — Fakan S, Odartchenko N Ultrastructural organization of the cell nucleus in early mouse embryos.

Hoppe-Seylers Z Physiol Chem — I Mitteilung. Biol Chem — Cerebellum — Int Rev Cytol — Experientia Reprod Domest Anim — Hayat H Stains and cytochemical methods. Plenum, New York. Hernandez-Verdun D, Derenzini M Non-nucleosomal configuration of chromatin in nucleolar organizer regions of metaphase chromosomes in situ. Eur J Cell Biol — Chromosoma — Bot Gaz — BioResources 14 3 — Jones ME Albrecht Kossel, a biographical sketch.

Yale J Biol Med — J Biol Chem — Methods Mol Biol — Kasten FH Schiff-type reagents in cytochemistry. Z Zellforch Microsk Anat Histochem — Kjellstrand PT Temperature and acid concentration in the search for optimum Feulgen hydrolysis conditions. Kobayashi S, Saio M, Fukuda T, Kimura K, Hirato J, Oyama T Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma.

Oncol Rep — Lessler MA Some quantitative aspects of the Feulgen reaction for desoxyribose nucleic acid. Science — Levene A Hydrolysis of yeast nucleic acid with dilute alkali at room temperature conditions of Steudel and Peiser. Article PubMed Google Scholar. Tracing nascent DNA by bromodeoxyuridine into structures arising during the S phase.

Acta Histochem — Medicinisch-Chemische Untersuchungen — Moyne G Methods in ultrastructural cytochemistry of the cell nucleus. Prog Histochem Cytochem — An ultracytochemical and autoradiographical study. J Gen Vrol — Acta Cytol — Chromosoma 6 2 — Peters D Electron microscopic studies on the localization of deoxyribonucleic acid inside of DNA viruses.

Maruzen, Tokyo, pp — Pourshahian S Therapeutic oligonucleotides, impurities, degradants, and their characterization by mass spectrometry. Mass Spec Rev — Retrieved January 09, from Encyclopedia. Then, copy and paste the text into your bibliography or works cited list. Because each style has its own formatting nuances that evolve over time and not all information is available for every reference entry or article, Encyclopedia.

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Biography of robert feulgent

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